Cyclo (Tyrosyl-Prolyl) Produced by Streptomyce sp.: Bioactivity and Molecular Structure Elucidation  

Oleh:

Sunaryanto, Rofiq ; MARWOTO, BAMBANG ; Hartoto, Liesbetini ; Mas'ud, Zainal Alim ; Irawadi, Tun Tedja

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 5 no. 2 (Jun. 2011), page 81-87.
Topik: Antibiotic; Cyclo (tyrosyl-prolyl); Inhibitory Concentration; Bio-activity
Fulltext: 5_her.pdf (321.44KB)

Isi artikel

Determination of bio-activity by minimum inhibitory concentration (MIC) methods and molecular structure identification of antibiotic produced by Streptomyces sp. have been carried out. The antibiotic was produced by liquid culture using Streptomyces sp. isolate. Purification of antibiotic was carried out by silica gel column chromatography and preparative HPLC. Molecular structure identification was carried out using ESI-MS, H NMR, C NMR, and C DEPT NMR. Pure antibiotic showed inhibition activity to Gram-negative and Gram-positive bacteria. MIC to Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC27853 , Staphylococcus aureus ATCC25923, and Bacillus subtilis ATCC66923 were 27.0, 68.7, 80.2, and 73.7 ìg mL , respectively. Identification using ESI-MS showed that the molecular weight of this antibiotic was 260 g mol , and molecular formula was C H N O . Elucidation of molecular structure using HNMR, C NMR, and C DEPTNMRshowed that antibiotic was cyclo(tyrosyl-prolyl).

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