Microarray expression profiling in adhesion and normal peritoneal tissues  

Oleh:

Ambler, Dana R. ; Golden, Alicia M. ; Gell, Jennifer S. ; Saed, Ghassan M.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 97 no. 05 (May 2012), page 1158-1164.
Topik: GENETICS; Postoperative adhesions; peritoneum; microarray; gene expression

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2012.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To identify molecular markers associated with adhesion and normal peritoneal tissue using microarray expression profiling. Design Comparative study. Setting University hospital. Patient(s) Five premenopausal women. Intervention(s) Adhesion and normal peritoneal tissue samples were obtained from premenopausal women. Ribonucleic acid was extracted using standard protocols and processed for hybridization to Affymetrix Whole Transcript Human Gene Expression Chips. Microarray data were obtained from five different patients, each with adhesion tissue and normal peritoneal samples. Real-time polymerase chain reaction was performed for confirmation using standard protocols. Main Outcome Measure(s) Gene expression in postoperative adhesion and normal peritoneal tissues. Result(s) A total of 1,263 genes were differentially expressed between adhesion and normal tissues. One hundred seventy-three genes were found to be up-regulated and 56 genes were down-regulated in the adhesion tissues compared with normal peritoneal tissues. The genes were sorted into functional categories according to Gene Ontology annotations. Twenty-six up-regulated genes and 11 down-regulated genes were identified with functions potentially relevant to the pathophysiology of postoperative adhesions. We evaluated and confirmed expression of 12 of these specific genes via polymerase chain reaction. Conclusion(s) The pathogenesis, natural history, and optimal treatment of postoperative adhesive disease remains unanswered. Microarray analysis of adhesions identified specific genes with increased and decreased expression when compared with normal peritoneum. Knowledge of these genes and ontologic pathways with altered expression provide targets for new therapies to treat patients who have or are at risk for postoperative adhesions.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali