Octanoate and Nonaoate Oxidation Increases 50–80% Over the First Two Days of Life in Piglet Triceps Brachii and Gracilis Muscle Strips  

Oleh:

Tetrick, Mark A. ; Crenshaw, Thomas D. ; Benevenga, Norlin J.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: JN: The Journal of Nutrition vol. 142 no. 06 (Jun. 2012), page 999-1003.
Topik: NUTRIENT; Nutrient Physiology; Metabolism; Nutrient-Nutrient Interactions

Ketersediaan

Perpustakaan FK Nomor Panggil: J42.K.2012.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

An in vitro muscle strip incubation system was developed to measure the rate of catabolism of 1 mmol/L [1-14C]octanoate, 1 mmol/L [1-14C]nonanoate, 1 mmol/L [9-14C]nonanoate, and 10 mmol/L [U-14C]glucose by measuring the recovery of 14CO2. Muscle strips (13 mm × 1.5 mm, ~50 mg) were isolated from triceps brachii and gracilis muscles of newborn and 2-d-old, small (<950 g) and large (>1450 g) piglets. The position of the 14C label in the substrate affected the rate and amount of recovery in 14CO2. Therefore, comparisons were made between age groups (0 vs. 2 d old) within substrates but limited across substrates to comparisons of [1-14C]–labeled fatty acids. The medium-chain fatty acid (MCFA) oxidation rates [pmol/(h?·?mg)] in muscle strips isolated from piglets from the 2 weight groups (<950 and >1450 g) did not differ (P > 0.99), there was a trend towards a difference between triceps brachii and gracilis muscle (P = 0.09; data not shown), and there were no significant interactions involving pig weight or muscle type; therefore, results were pooled across these factors. During the first 2 d of life, MCFA oxidation [pmol/(h?·?· mg muscle strip)] increased (P < 0.05) 50–80%, but the glucose oxidation rate did not change (P > 0.82). By d 2, the oxidation rate of nonanoate as represented by the one carbon was 25% greater than for octanoate (P < 0.05). The conversion of [9-14C]nonanoate to 14CO2 indicated that muscle had the capacity to oxidize the propionyl-CoA produced by ß-oxidation of nonanoate and that odd-chain C-9 MCFA provided anabolic carbon to the citric acid cycle.

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