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Antifungal Peptides from a Burkholderia Strain Suppress Basal Stem Rot Disease of Oil Palm (article of Phytopathology Feb 2022 Vol.112 Iss.2 p.238-248)
Bibliografi
Author:
Prihatna, Cahya
;
Pramudito, Theodorus Eko
;
ARIFIN, ARILD RANLYM
;
Thi Kim Ngan Nguyen
;
Purnamasari, Maria Indah
;
Suwanto, Antonius
Topik:
BACTERIOLOGY BIOCHEMISTRY AND CELL BIOLOGY BIOLOGICAL CONTROL BIOTECHNOLOGY DISEASE CONTROL AND PEST MANAGEMENT FUNGAL PATHOGENS
;
JABFUNG-FTB-TEP-2023
Bahasa:
(EN )
Penerbit:
The American Phytopathological Society (APS)
Tempat Terbit:
Minnesota, USA
Tahun Terbit:
2022
Jenis:
Article - diterbitkan di jurnal ilmiah internasional
Fulltext:
B4 Antifungal Peptides from a Burkholderia Strain Suppress Basal Stem Rot Disease of Oil Palm.pdf
(2.49MB;
1 download
)
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Abstract
Basal stem rot (BSR) is the most common disease of oil palm (Elaeis guineensis) in Southeast Asia. BSR is caused by a white-rot fungus Ganoderma boninense. The disease is difficult to manage. Therefore, development of novel and environmentally safe approaches to control the disease is important. Species of Burkholderia are known to have diverse lifestyles, some of which can benefit plants by suppressing diseases or increasing plant growth. In the present study, antifungal peptides produced by a bacterial strain isolated from the rhizosphere of an oil palm tree, Burkholderia sp. strain CP01, exhibited strong growth inhibition on G. boninense. A loss-of-function mutant of CP01 was generated, and it has enabled the identification of a 1.2-kDa peptide and its variants as the active antifungal compounds. High-resolution mass spectrometry revealed six analogous compounds with monoisotopic masses similar to the previously reported cyclic lipopeptides occidiofungin and burkholdine. The antifungal compounds of CP01 were secreted into media, and we sought to use CP01 culture extract without living cells to control BSR disease. Glasshouse experiments showed that CP01 culture extract suppressed BSR disease in oil palm seedlings. The ability of CP01 to produce an antifungal substance and suppress plant disease suggests its potential applications as a biofungicide in agriculture.
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