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Positive correlation between the number of bacteria in soybean tempeh with the bioactivity of its extract against enterotoxigenic Escherichia coli (ETEC) adhesion to eukaryotic cells (article of Food Research Vol.5 Iss.6 p.157- 164 December 2021)
Bibliografi
Author:
Pramudito, Theodorus Eko
;
Florencia, G.
;
Gunawan, M.R.
;
Pratiwi, M.P.
;
Yogiara
Topik:
Tempeh
;
Diarrhea
;
Enterotoxigenic Escherichia coli (ETEC)
;
Bacteria
;
Yeast agglutination assay
;
Anti-adhesion bioactivity
;
JABFUNG-FTB-TEP-2023
Bahasa:
(EN )
Penerbit:
Rynnye Lyan Resources
Tahun Terbit:
2021
Jenis:
Article - diterbitkan di jurnal ilmiah internasional
Fulltext:
_20__fr-2021-110_pramudito_1.pdf
(467.07KB;
1 download
)
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Abstract
Bioactive oligosaccharides from soybean tempeh can inhibit the adhesion of
enterotoxigenic Escherichia coli (ETEC) to intestinal cells, thus reducing the severity of
ETEC-mediated diarrhea. Bacteria are also present in tempeh but there has yet been any
report regarding their effect on the anti-adhesion bioactivity of tempeh. In this research,
the bacterial number in tempeh was quantified and the anti-adhesion bioactivity of tempeh
extract was determined using yeast agglutination assay. Statistical analysis showed a
moderately (R = 0.69) significant positive correlation (P < 0.01) between the number of
bacteria in tempeh and the anti-adhesion bioactivity of its extract. In conclusion, tempeh
that contains more bacteria is more effective in inhibiting ETEC adhesion to eukaryotic
cells. This could be due to a symbiosis between Rhizopus and bacteria in breaking down
soy matrix polysaccharides to release bioactive oligosaccharides.
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