Biofilm involvement in chronic infections and on the surface of medical equipment have been considered as
public health concern worldwide. Bacterial biofilm is related to antibiotic resistance, making the diseases
difficult to treat. An effective control strategy should be implemented, for example, by applying antibiofilm
agents. The use of aquatic environment as potential sources of bioactive compounds, including the
antibiofilm compounds, is recently of concern. This study aimed to screen and characterize bacteria with
antibiofilm activity that were isolated from waterfall and marine environment and obtained from several
locations in Indonesia. The isolates were firstly evaluated for their antimicrobial activity against six
bacterial pathogens and followed by antibiofilm screening. Eleven out of 65 isolates showed quorum sensing
or quorum quenching activity, and one of them showed both activities. Supernatants of 11 isolates inhibited
biofilm formation of at least one pathogen by using static biofilm assay. Bioactive compounds
characterization of the selected five isolates revealed the presence of different compounds, such as
carbohydrates, proteins, and nucleic acids. The 16S rRNA gene sequencing analysis classified five isolates
into two different genera, namely Vibrio (WK2.4, WK2.6, and WK2.3) and Pseudomonas (S1.2 and S1.3).
The present study provides insights into the discovery of aquatic bacteria candidates as antibiofilm agents. |