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Screening of secondary metabolite from waterfall and marine bacteria as biocontrol agent (article of International Research Journal of Microbiology (IRJM) Vol.7(1) pp.001-009, February, 2018)
Bibliografi
Author:
Magdalena, Stella
;
Marlim, Clarisa
Topik:
Marine bacteria
;
Waterfall
;
Antimicrobial
;
Biocontrol agent
;
Brine shrimp
;
JABFUNG-FTB-STM-2023-06
Bahasa:
(EN )
Penerbit:
International Research Journals
Tahun Terbit:
2018
Jenis:
Article - diterbitkan di jurnal ilmiah internasional
Fulltext:
B6_Artikel IRJM.pdf
(338.8KB;
1 download
)
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Abstract
Biocontrol agent often used to reduce sources of disease, such as bacteria, fungi, and mosquito.
Emergence of antibiotic-resistant bacteria and fungi are increasing due to excessive used of
antimicrobial substances. Chemical substances used to kill mosquito larvae in water environment
might also be dangerous for human and environment. Hence, a biocontrol agent is required to be safe
and effective. The purpose of this study is to discover bacteria from waterfall and marine water which
has a good potential as biocontrol agent. Four waterfall bacteria (S 1.1, S 2.1, S 3.1, and S 3.2) and three
marine bacteria (A 1.1, A 1.2, and PA 1.3C) were proved to have antifungal activity towards pathogenic
fungal. PA 1.3A was the only marine bacteria which proved to have antimicrobial activity towards
Escherichia coli and Streptococcus agalactiae. Extraction of antimicrobial substance using several
solvents resulted in larger clear zone diameter and inhibition of other bacteria, such as Bacillus
licheniformis and Staphylococcus aureus. Extracted antimicrobial substance of PA 1.3A was also
proved to be non toxic towards Aedes aegypti larvae through larvicidal assay and mammals through
brine shrimp lethality assay (LC50 > 1000µg/mL).
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