Detail Isolation and Evaluation of Probiotic Potential of Lactic Acid Bacteria from Oncom (presented at 10th Asian Conference on Lactic Acid Bacteria) Bibliografi Author: Magdalena, Stella ; Jennifer, Fransisca ; Yogiara Topik: JABFUNG-FTB-STM-2023-15 Bahasa: (EN )     Tahun Terbit: 2019     Jenis: Poster Fulltext: B15_Poster BAL ACLAB 2019.pdf (912.33KB; 3 download) Abstract Oncom is one of Indonesian traditional fermented food which made by fermentation of various species of fungi, Neurospora intermedia var. oncomensis and Rhizopus oligosporus. Diversity of microorganism in the consortium has not been thoroughly explored and studied. The aim of the present work was to isolate and examine the potential probiotic properties of lactic acid bacteria (LAB) from oncom, such as production of antibacterial substances, haemolytic activity, low pH tolerance, and bile resistance of LAB isolated. From two red oncom and one black oncom, 11 LAB isolates were isolated and five selected isolates were identified by 16S rDNA sequencing as Pediococcus pentosaceus (LR.JBb.1; LR.C.27; LB.Boe.34; LB.Boe.36) and Lactobacillus plantarum (LB.Boe.35). In general, all identified LAB were able to inhibit both Escherichia coli and Staphylococcus aureus. Antibacterial against Salmonella enterica was absent in LB.Boe.34 and LB.Boe.36. Alphahaemolytic activity was observed in LR.C.27, LB.Boe.34, and LB.Boe.35. In general, the selected strains showed moderate to tolerate the acidity at pH 2.5 after 3 hours of incubation, but no tolerance to pH 1.0. LAB isolates were able to survive in the presence of 0.3% bile. These findings imply that Pediococcus pentosaceus LR.JBb.1 and LR.C.27 appeared as the promising candidates to be used as potential probiotics and might be subjects for in vivo studies. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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