Detail Isolation and in vitro Sreening of Beficial Endophyte Bacteria against Fusarium wilt in banana (Musa acuminata AAA group cv. Pisang ambon kuning) (article in Proceeding of International Conference on Life Science and Biological Engineering (LSBE) at Nagoya, Japan 4-6 November 2015) Bibliografi Author: Karmawan, Listya Utami ; Dwivany, Fenny Martha ; Aryantha, I Nyoman Pugeg Topik: In vitro screening; plant fitness and defense system; necrosis; medium browning; relative growth rate; JABFUNG-FTB-LUK-2022 Bahasa: (EN )     Penerbit: Higher Education Forum     Tempat Terbit: Nagoya    Tahun Terbit: 2015     Jenis: Others (lainnya) Fulltext: 2015_LSBE_Conference Proceedings_LUK.pdf (1.86MB; 4 download) Abstract Fusarium wilt which caused by fungi Fusarium oxysporum has threaten the world banana production including Indonesia. This become a serious drawback since banana is very common food commodity in Indonesia as well as in the world. Conventional management of Fusarium wilt was focused on fumigation and finding new resistant cultivar of banana. Yet, research about beneficial endophyte bacteria which has antagonistic properties against pathogen or enhancing plant defense system was neglected until recent decades. Fourteen endophyte isolates from local plantation in Buleleng, Bali, Indonesia were screened using in vitro triple culture assay. Endophyte bacteria were inoculated to banana plantlet to enhance plant fitness and defense system. Seven days after inoculation, Fusarium oxysporum f.sp. cubense tropical race 4 (Foc4) was added to the in vitro system and co-cultured for two another weeks. Total necrosis area, shoot necrosis area, medium browning index, and Relative Growth Rate (RGR) at 14 days after Fusarium inoculation were analyzed. Pictures were analyzed digitally using ImageJ 1.48v software. Statistical analysis showed that isolate 8b (putatively Ochrobactrum tritici) significantly reduced total necrosis area (23.25%) and shoot necrosis area (21.49%) compared to control (44.25% and 42.58% respectively). Isolate 25a.2 (putatively Bacillus aerius) significantly showed a lower medium browning index (index score 0.40) compared to control (index score 1.52). There was no significant difference for RGR, nevertheless banana plantlet inoculated with isolate 9d (putatively Bacillus pseudomycoides) showed the highest RGR (97.33 mg/days). The ability of these endophyte bacteria to decrease necrosis and medium browning of banana plantlet during Fusarium infection could lead to higher plant survival against Fusarium wilt. Further analysis will be done to confirm beneficial properties of these bacterial endophyte isolates. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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