Cloning and expression of pab gene of Mycobacterium tuberculosis isolated from pulmonary TB patient in Escherichia coli DH5  

Oleh:

Raras, Tri Yudani. M. ; Lyrawati, Diana

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Medical Journal of Indonesia vol. 20 no. 04 (Nov. 2011), page 247-254.
Fulltext: Volume 20, Issue 4, November 2011 - Cloning and expression of pab gene of Mycobacterium tuberculosis isolated from pulmonary TB patient in Escherichia coli DH5.pdf (3.92MB)

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Perpustakaan FK Nomor Panggil: M35.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

ABSTRACT Aim Mycobacterium tuberculosis antigen 38 is a potent serodiagnostic agent containing two M. tuberculosis-specific B-cell epitopes. The high price of imported diagnostic agents hinders realization of fast clinical TB diagnosis in developing countries. Therefore, we produced recombinant antigen 38 (recAg38M) from M. tuberculosis local strain, which might be used to produce economical tuberculosis serodiagnostic kit. Method pab gene that was isolated from pulmonary TB patient in Malang was cloned into a plasmid vector (pGem-T Easy) to construct pMB38. The E. coliDH5??clone carrying pMb38 was selected on X-gal medium. The overexpression of pab was mediated using pPRoExHTc under the control of Trc promoter and Escherichia coliDH5? as host. Results Alignment of the pab sequence from the white E. coliDH5??clones with that of M. tuberculosisH37Rv showed 98% homology. The recombinant protein in which the signal peptide has been deleted to prevent the protein being secreted into medium was found in the cytoplasm. Conclusion pab gene from Malang could be successfully expressed in heterologous system intracellularly.

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