Iliac crest and femoral bone marrow as the source of plastic-adherent cells  

Oleh:

Phedy ; Dilogo, Ismail H. ; Jusuf, Ahmad A. ; Kholinne, Erica ; Efendi, Zuhri

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Medical Journal of Indonesia vol. 20 no. 02 (May 2011), page 100-104.
Topik: Plastic-Adherent Cells; Red Marrow; Yellow Marrow
Fulltext: Volume 20, Issue 2, May 2011 - Iliac crest and femoral bone marrow as the source of plastic-adherent cells.pdf (144.86KB)

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Perpustakaan FK Nomor Panggil: M35.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Background: Many studies have used iliac crest as the source of mesenchymal stem cells. In cases of long bone shaft fracture, obtaining marrow from the fracture site offers more advantages. Nevertheless, due to the high number of fat cells in long bones, the yellow marrow of long bones is believed to contain lower number of mesenchymal stem cells than red marrow. Therefore the aim of this study is to compare the potency between red and yellow marrow as the donor site for the isolation of mesenchymal stem cell. Methods: Bone marrow of eight giant Flemish rabbits was aspirated from the iliac crest and femoral shaft. Mononuclear cells were isolated from both aspirates and expanded in low glucose DMEM. After eight weeks, the cells were harvested and counted using improved Neubauer hemocytometer. Comparison of the cell number between the two donor sites was then performed by t-test. Results: After 8 weeks, an average number of 2.93±0.91 x 104 and 3.7±2.50 x 104 cells were obtained from the iliac and femoral group respectively. No statistically signifi cant difference was found between those two groups (p= 0.45). Conclusion: Plastic-adherent cells can be isolated and expanded from both iliac crest and femoral shaft.

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