Phospholipase C-?-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection  

Oleh:

Swann, Karl ; Windsor, Shane ; Campbell, Karen E. ; Elgmati, Khalil

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 97 no. 03 (Mar. 2012), page 742-747.
Topik: Oocyte; Zygote; Calcium; Movement; Phospholipase C Zeta; Cross Correlation; Particle Image Velocimetry

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2012.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To evaluate the imaging of cytoplasmic movements in human oocytes as a potential method to monitor the pattern of Ca2+ oscillations during activation. Design Test of a laboratory technique. Setting University medical school research laboratory. Patient(s) Donated unfertilized human oocytes from intracytoplasmic sperm injection (ICSI) cycles. Intervention(s) Microinjection of oocytes with phospholipase C (PLC) zeta (?) cRNA and a Ca2+-sensitive fluorescent dye. Main Outcome Measure(s) Simultaneous detection of oocyte cytoplasmic movements using particle image velocimetry (PIV) and of Ca2+ oscillations using a Ca2+-sensitive fluorescent dye. Result(s) Microinjection of PLC? cRNA into human oocytes that had failed to fertilize after ICSI resulted in the appearance of prolonged Ca2+ oscillations. Each transient Ca2+ concentration change was accompanied by a small coordinated movement of the cytoplasm that could be detected using PIV analysis. Conclusion(s) The occurrence and frequency of cytoplasmic Ca2+ oscillations, a critical parameter in activating human zygotes, can be monitored by PIV analysis of cytoplasmic movements. This simple method provides a novel, noninvasive approach to determine in real time the occurrence and frequency of Ca2+ oscillations in human zygotes.

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