Detail Rhizopus Rotting on Agricultural Products in Jakarta (article of Hayati Journal of Biosciences Vol. 27 No. 1 January 2020) Bibliografi Author: Hartanti, Anastasia Tatik ; Raharjo, Amelia ; Gunawan, Agustin Wydia Topik: Rhizopus rot; ITS rDNA; mold; postharvest disease; agriculture Bahasa: (EN )     Penerbit: Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University     Tempat Terbit: Bogor    Tahun Terbit: 2020     Jenis: Article - diterbitkan di jurnal ilmiah nasional Fulltext: 1-30309-Article Text-99458-1-10-20200408.pdf (417.36KB; 0 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract Agricultural products may easily perish if they are not well taken care of during postharvest treatment. One of the main causes in damaged products is the biological contamination of pathogenic fungi, such as Rhizopus spp. that gives rise to Rhizopus rot symptoms. The purpose of this research was to isolate Rhizopus spp. from different agricultural products showing Rhizopus rot symptoms as well as to identify them. Rhizopus pure cultures were isolated on potato dextrose agar. Identification was done through molecular techniques using PhytopureTM DNA Extraction Kit and Maxwell® RSC Plant DNA Kit for DNA isolation, internal transcribed spacer (ITS4 and ITS5) as primers for amplification, and molecular evolutionary genetics analysis 7 (MEGA7) for the reconstruction of phylogenetic tree from sequence result. The phylogenetic tree using Maximum Likelihood statistics with 1,000 replications of bootstrap test showed five strains, namely AR9, AR10, AR11, AR13, and AR14, which belong to R. delemar, and the other seven remaining strains, AR1-AR7 belong to R. stolonifer. Identification was clarified with morphological and physiological data using Rhizopus growth at temperature control of 33 and 42°C as well as microscopic observation involving rhizoid, columella, measurement of sporangiospores, and sporangiophores. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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