An imbalance in interleukin-17-producing T and Foxp3+ regulatory T cells in women with idiopathic recurrent pregnancy loss  

Oleh:

Lee, S.K. ; Kim, J.Y. ; Hur, S.E. ; Kim, C.J.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Human Reproduction vol. 26 no. 11 (Nov. 2011), page 2964-2971.
Topik: Interleukin-17; Th17; Foxp3; Treg; recurrent pregnancy loss

Ketersediaan

Perpustakaan FK Nomor Panggil: H07.K.2011.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

BACKGROUND T cells which produce interleukin (IL)-17 are involved in chronic inflammatory processes and regulatory T (Treg) cells are possibly the most important immune regulators. We aimed to investigate peripheral blood IL-17+ T and Foxp3+ Treg cells in women with idiopathic recurrent pregnancy loss (RPL). METHODS The study design is a cross-sectional evaluation of Th1, Th2, IL-17+ T and Treg cells in women with idiopathic RPL (n = 42) and age-matched parous controls (n = 24). Flow cytometric analysis was performed to measure IL-17+ T and Foxp3+ Treg cells, and ratios of Th1/Th2 cells using anti-IL-17A and anti-Foxp3 antibodies, and monoclonal antibodies to tumor necrosis factor (TNF)-a, interferon (IFN)-? and IL-10. Student's t-test and partial correlations were applied for statistical analysis. RESULTS TNF-a-/IL-10-producing CD3+CD4+ T cell ratio was higher in women with RPL than controls (P = 0.048). Levels of IL-17+ T cells (P = 0.021) and the IL-17+ T/CD4+Foxp3+ Treg cell ratio (P = 0.001) were increased, whereas Foxp3+ (P = 0.035), Foxp3low (P = 0.032) and CD4+Foxp3+ T cell (P = 0.037) levels were decreased in women with RPL, compared with controls. Levels of IL-17+ T cells were correlated with TNF-a-producing CD3+CD4+ T cells (r = 0.269, P = 0.033), and with ratios of TNF-a/IL-10 (r = 0.276, P = 0.027) and IFN-?/IL-10 (r = 0.266, P = 0.035)-producing CD3+CD4+ cells. Furthermore, the ratio of IL-17+ T cells to CD4+Foxp3+ Treg cells showed a positive correlation with TNF-a-producing CD3+CD4+ T cells (P = 0.047) and IFN-?-producing CD3+CD4+ T cells (P = 0.048) as well as a ratio of IFN-?/IL-10-producing CD3+CD4+ T cells (P = 0.037). CONCLUSIONS Enhanced pro-inflammatory immune responses with suppressed immune regulation may be an important immune mechanism involved in RPL.

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