Hyperthyroidism advances luteolysis in the pregnant rat through changes in prostaglandin balance  

Oleh:

Navas, Paola B. ; Motta, Alicia B. ; Hapon, María B. ; Jahn, Graciela A.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 96 no. 04 (Oct. 2011), page 1008-1014.
Topik: Pregnancy; P; luteolysis; PGF2a; PGE2; PRL; receptors; 20aHSD

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To investigate the underlying mechanisms implicated in the premature luteolysis induced by hyperthyroidism in pregnant rats. Design Experimental basic study. Setting Research institute. Animal(s) Groups of 6–8 adult female Wistar rats were injected SC daily with T4 (0.25 mg/kg) or vehicle, starting 8 days before mating, and killed by decapitation on days 19 (G19), 20 (G20), and 21 (G21) of pregnancy. Intervention(s) Corpora lutea and truncal blood of control and hyperthyroid rats were obtained. Main Outcome Measure(s) Circulating and intraluteal hormones were determined by using RIA and luteal messenger RNA (mRNA) expression of enzymes and factors involved in P synthesis and metabolism by reverse transcriptase–polymerase chain reaction. 20a-Hydroxysteroid dehydrogenase (20aHSD) mRNA and protein expression was also determined by quantitative reverse transcriptase–polymerase chain reaction and Western blot. Result(s) Hyperthyroidism advanced luteolysis and 20aHSD expression induction by one day without changes in enzymes involved in P synthesis, decreased circulating E2 and luteal estrogen receptor beta, and increased luteal prostaglandin F2a on G19 and G20 and prostaglandin E2 on G19, while decreasing it on G20. Thus, decreased estrogenic influence and high prostaglandin F2a/prostaglandin E2 ratio favors premature induction of 20aHSD on hyperthyroid rats. Conclusion Hyperthyroidism affects luteolysis in pregnant rats through alterations in luteal prostaglandin balance and decreased luteotrophic factors favoring the luteolytic action of prostaglandin F2a that induces premature 20aHSD expression that in turn advances circulating P fall and delivery.

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