Assessment of 1,2-propanediol (PrOH) genotoxicity on mouse oocytes by comet assay  

Oleh:

Berthelot-Ricou, Anais ; Perrin, Jeanne ; Giorgio, Carole Di ; de Meo, Michel

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 96 no. 04 (Oct. 2011), page 1002-1007.
Topik: 1; 2-Propanediol; PrOH; cryoprotectant; mouse; oocytes; genotoxicity; DNA damage; comet assay

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To assess the genotoxicity of 1,2-propanediol (PrOH) on mouse oocytes by comet assay. Design In vitro assay using murine model. Setting Biogenotoxicology research laboratory. Animal(s) CD1 female mice. Intervention(s) Three 40-oocyte groups were exposed to different PrOH concentrations (5%, 7.5%, and 15%). Each concentration was tested during both long and short exposures (1–2 hours and 1–5 minutes) in comparison with control groups. DNA damage was evaluated by a single-cell gel electrophoresis assay, also called “comet assay,” and analyzed with Komet software. Main Outcome Measure(s) DNA damage was quantified as Olive tail moment (OTM). Interpretation was done on OTM with the use of ?2. Result(s) High PrOH concentrations (7.5% and 15%) induced significant DNA damage on mouse oocytes. The OTM ?2 values were 4.16 ± 0.40 and 6.80 ± 0.4 with 7.5% PrOH at 1 and 2 hours, respectively, 24.35 ± 1.60 with 15% at 1 hour, and for 2h at 15% the DNA damage was too drastic to calculate OTM ?2. After 1 and 5 minutes, the OTM ?2 values were, respectively, 5.19 ± 0.26 and 6.06 ± 0.42 with 7.5%, and 7.53 ± 0.33 and 16.81 ± 0.67 with 15%. Conclusion(s) High concentrations of PrOH (7.5% and 15%) induced significant DNA damage on mouse oocytes, whatever the exposure duration. These results should be interpreted with caution, because additional data are needed to evaluate PrOH genotoxicity and DNA oocyte reparation after exposure to high PrOH concentrations.

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