Human chorionic gonadotropin from day 2 spent embryo culture media and its relationship to embryo development  

Oleh:

Ramu, Sivakumar ; Acacio, Brian ; Adamowicz, Mark ; Parrett, Sylvia

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 96 no. 03 (Sep. 2011), page 615-617.
Topik: hCG; embryo development; ELISA; spent culture media

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To detect hCG in spent embryo culture media at day 2 after intracytoplasmic sperm injection and to assess the relationship of hCG to embryo development. Design Experimental study. Setting Fertility center and clinical diagnostic laboratory. Sample(s) A total of 102 spent culture media from day 2 human embryos and corresponding unexposed media for blank control. Intervention(s) The culture media samples were tested for hCG by ELISA. Main Outcome Measure(s) Quantity of hCG produced by embryos and correlation with the embryos’ developmental status. Result(s) hCG was found in 93 of 102 culture media tested by enzyme-linked immunosorbent assay. The correlation analysis revealed that the concentration of hCG was independent of embryo developmental status. Conclusion(s) The ability to detect hCG from day 2 spent culture media may be used as a marker for embryo competence.

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