Expression of dysadherin in the human male reproductive tract and in spermatozoa  

Oleh:

Gabrielli, Nieves Maria ; Veiga, Maria Florencia ; Matos, Maria Laura ; Quintana, Silvina

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 96 no. 03 (Sep. 2011), page 554-561.
Topik: Spermatozoa; dysadherin; epithelial cadherin; Na+; K+-ATPase; a4-subunit

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To study expression of dysadherin in human testis, epididymis, and spermatozoa. Design Prospective study. Setting Basic research laboratory. Patient(s) Testis, epididymis, and testicular spermatozoa from patients under treatment and semen from volunteer donors. Intervention(s) Reverse transcription–polymerase chain reaction, immunohistochemistry, immunocytochemistry, and Western immunoblotting. Main Outcome Measure(s) Dysadherin messenger RNA (mRNA) analysis in testis, epididymis, and ejaculated spermatozoa, immunohistochemistry of both tissues, Western immunoblotting of tissue/cell extracts, and immunocytochemistry of spermatozoa. Result(s) Dysadherin mRNA was found in testis, epididymis, and ejaculated spermatozoa. Whereas testis and spermatozoa exhibited a distinctive 91-kDa protein form, the epididymis showed a 50-kDa moiety, also found in MDA-MB-231 breast cancer cells. Nucleotide sequence analysis revealed >99% homology between testicular and somatic cell mRNA, suggesting differential protein glycosylation. Dysadherin was immunodetected in round spermatids and testicular/ejaculated spermatozoa. It localizes to the acrosomal region and flagellum and colocalized with E-cadherin in the head and with the Na+,K+-ATPase a4 subunit in the flagellum. Conclusion(s) This is the first report on expression of dysadherin in the male gonad and in spermatozoa. Its colocalization with E-cadherin and Na+,K+-ATPase leads us to postulate a role for dysadherin as a modulator of sperm function.

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