Activin-A is induced by interleukin-1ß and tumor necrosis factor-a and enhances the mRNA expression of interleukin-6 and protease-activated receptor-2 and proliferation of stromal cells from endometrioma  

Oleh:

Yoshino, Osamu ; Izumi, Gentaro ; Shi, Jia ; Osuga, Yutaka

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 96 no. 01 (Jul. 2011), page 118-121.
Topik: Endometriosis; activin-A; inflammatory cytokine; IL-6; PAR-2

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To examine the regulation and the function of activin-A in stromal cells derived from endometrioma. Design Molecular studies. Setting University research laboratory. Patient(s) Endometrioma stromal cells (EoSC) were obtained from 28 patients with ovarian endometrioma undergoing laparoscopy. Intervention(s) EoSC were cultured with inflammatory stimuli or recombinant activin-A, followed by RNA extraction. Main Outcome Measure(s) Activin mRNA expression was evaluated by real-time reverse transcription–polymerase chain reaction (RT-PCR), and activin-A concentration of supernatant of cultured EoSC was evaluated by ELISA. Also, the effect of activin-A on EoSC was evaluated with real-time RT-PCR and cell proliferation assay. Result(s) Inflammatory stimuli, interleukin (IL) -1ß, and tumor necrosis factor (TNF) -a induced inhibin/activin-ßA subunit mRNA and activin-A protein expression in EoSC. Additionally, activin-A enhanced EoSC proliferation and increased the expression of IL-6 and protease-activated receptor (PAR)-2 mRNA. Conclusion(s) An in vitro study revealed that activin-A, which is induced by IL-1ß or TNF-a, might promote endometriosis by stimulating IL-6 and PAR-2 mRNA expression and increasing the proliferation of EoSC.

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