Melatonin protects human spermatozoa from apoptosis via melatonin receptor– and extracellular signal–regulated kinase-mediated pathways  

Oleh:

Espino, Javier ; Ortiz, Agueda ; Bejarano, Ignacio ; Lozano, Graciela M.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 95 no. 07 (Jun. 2011), page 2290-2296.
Topik: Melatonin; apoptosis; spermatozoa; MT receptor; MAPK signaling

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To evaluate whether the protective effect of melatonin on H2O2-induced caspase activation and DNA fragmentation depends on the interaction between melatonin and its surface receptors. Design Laboratory study. Setting Center for assisted human reproduction at a Spanish hospital. Patient(s) Twenty-one healthy donors. Intervention(s) Human spermatozoa were treated with increasing concentrations of hydrogen peroxide (H2O2; 1µM, 10 µM, 100 µM, 1mM) and preincubated with 1 mM melatonin. Main Outcomes Measure(s) Activation of caspase-3 and -9 as well as DNA fragmentation were examined by fluorescence methods. Result(s) Our findings showed that H2O2 induced a significant increase in caspase-9 and caspase-3, which was dose independent. Conversely, pretreatment with melatonin reduced H2O2-mediated caspase activation in a dose-dependent way. Moreover, the antiapoptotic effects of melatonin in ejaculated human spermatozoa may involve membrane melatonin receptor MT1. In addition, we found that the survival-promoting pathway extracellular signal–regulated kinase (ERK) is likely to have a role in the protective actions of melatonin in ejaculated human spermatozoa. Finally, we confirmed these results further by demonstrating that melatonin prevention of H2O2-induced DNA fragmentation is dependent on both MT1 receptor and ERK signaling. Conclusion(s) These results indicate that the stimulation with melatonin triggers a set of events culminating in cell death prevention in ejaculated human spermatozoa.

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