Quercetin is equally or more effective than resveratrol in attenuating tumor necrosis factor-a–mediated inflammation and insulin resistance in primary human adipocytes  

Oleh:

Chuang, Chia-Chi ; Martinez, Kristina ; Guoxiang, Xie ; Kennedy, Arion ; Bumrungpert, Akkarach ; Overman, Angel ; Wei, Jia ; McIntosh, Michael K.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The American Journal of Clinical Nutrition vol. 92 no. 06 (Dec. 2010), page 1511-1521 .
Topik: Plant Polyphenols; Human Adipocytes; Quercetin; Trans-resveratrol
Fulltext: Am J Clin Nutr-2010-Chuang-1511-21.pdf (872.84KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: A07.K.2010.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Background: Quercetin and trans-resveratrol (trans-RSV) are plant polyphenols reported to reduce inflammation or insulin resistance associated with obesity. Recently, we showed that grape powder extract, which contains quercetin and trans-RSV, attenuates markers of inflammation in human adipocytes and macrophages and insulin resistance in human adipocytes. However, we do not know how quercetin and trans-RSV individually affected these outcomes. Objective: The aim of this study was to examine the extent to which quercetin and trans-RSV prevented inflammation or insulin resistance in primary cultures of human adipocytes treated with tumor necrosis factor-a (TNF-a)—an inflammatory cytokine elevated in the plasma and adipose tissue of obese, diabetic individuals. Design: Cultures of human adipocytes were pretreated with quercetin and trans-RSV followed by treatment with TNF-a. Subsequently, gene and protein markers of inflammation and insulin resistance were measured. Results: Quercetin, and to a lesser extent trans-RSV, attenuated the TNF-a–induced expression of inflammatory genes such as interleukin (IL)-6, IL-1ß, IL-8, and monocyte chemoattractant protein-1 (MCP-1) and the secretion of IL-6, IL-8, and MCP-1. Quercetin attenuated TNF-a–mediated phosphorylation of extracellular signal–related kinase and c-Jun-NH2 terminal kinase, whereas trans-RSV attenuated only c-Jun-NH2 terminal kinase phosphorylation. Quercetin and trans-RSV attenuated TNF-a–mediated phosphorylation of c-Jun and degradation of inhibitory ?B protein. Quercetin, but not trans-RSV, decreased TNF-a–induced nuclear factor-?B transcriptional activity. Quercetin and trans-RSV attenuated the TNF-a–mediated suppression of peroxisome proliferator–activated receptor ? (PPAR?) and PPAR? target genes and of PPAR? protein concentrations and transcriptional activity. Quercetin prevented the TNF-a–mediated serine phosphorylation of insulin receptor substrate-1 and protein tyrosine phosphatase-1B gene expression and the suppression of insulin-stimulated glucose uptake, whereas trans-RSV prevented only the TNF-a–mediated serine phosphorylation of insulin receptor substrate-1. Conclusion: These data suggest that quercetin is equally or more effective than trans-RSV in attenuating TNF-a–mediated inflammation and insulin resistance in primary human adipocytes.

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