Expression of Aquaporin 4 and Kir4.1 in Diabetic Rat Retina: Treatment with Minocycline  

Oleh:

Y, Zhang ; G, Xu ; Q, Ling

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The Journal of International Medical Research vol. 39 no. 02 (Mar. 2011), page 464-479.
Topik: Skin Transplantation; Acute Graft Rejection; Major Histocompatibility Complex Class I; Cd3+Cd8+ T-Lymphocytes; Peripheral Blood; Protein; Flow Cytometry; Mouse Model

Ketersediaan

Perpustakaan FK Nomor Panggil: J11.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

This study examined aquaporin 4 (AQP4) and Kir4.1 (a potassium channel subunit) in normal and diabetic adult Sprague–Dawley rats, and determined the effect of minocycline treatment. Retinal expression of the AQP4 and Kir4.1 genes was examined using double immunofluorescence, Western blot analysis, and real-time reverse transcription–polymerase chain reaction. Retinal levels of vascular endothelial growth factor (VEGF), ionized calcium-binding adaptor molecule (Iba)-1 and interleukin (IL)-1b were also ascertained. The blood–retinal barrier (BRB) and retinal oedema were assessed using rhodamine isothiocyanate. AQP4, VEGF, Iba-1, and IL-1beta mRNA and protein levels increased, and Kir4.1 mRNA and protein levels decreased, in diabetic rat retinas. Both BRB disruption and retinal oedema were also observed in these retinas. In diabetic rats, minocycline treatment decreased AQP4, VEGF, Iba-1 and IL-1beta levels and retinal oedema, and increased Kir4.1 levels. These findings suggest that minocycline might be beneficial for retinal fluid clearance and reduction of retinal oedema in diabetic retinopathy.

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