Effects of Dicalcium Silicate Coating Ionic Dissolution Products on Human Mesenchymal Stem-cell Proliferation and Osteogenic Differentiation  

Oleh:

H-W, Li ; J-Y, Sun

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The Journal of International Medical Research vol. 39 no. 01 (Mar. 2011), page 112-128.
Topik: Dicalcium Silicate; Biomaterials; Bone Replacement; Joint Replacement; Mesenchymal Stem Cells; Proliferation; Osteogenesis
Fulltext: 1573.pdf (599.24KB)

Ketersediaan

Perpustakaan FK Nomor Panggil: J11.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

This study investigated the effects of ionic dissolution products released from dicalcium silicate (DS) coatings on human mesenchymal stem cells (hMSC), cultured in the presence or absence of the dissolution products, with or without osteogenic supplements (OS). DS+ medium promoted cell proliferation during the first 4 days, but then inhibited proliferation. DS+OS- medium increased alkaline phosphatase (ALP) activity on day 14, and upregulated runt-related transcription factor 2 and osteonectin mRNA on days 7 and 14, respectively. The addition of osteogenic supplements (DS+OS+) led to a significant increase in ALP activity from days 7 to 21, upregulation of osteogenic markers on day 14, and formation of more mineralized nodules on day 28. The results demonstrated that the ionic dissolution products from DS coating alone can partly induce osteogenic differentiation of hMSC, and that the addition of osteogenic supplements further enhances osteoblast-specific gene expression and mineralization in hMSC.

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