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Proteomics study of extracellular fibrinolytic proteases from Bacillus licheniformis RO3 and Bacillus pumilus 2.g isolated from Indonesian fermented food (article of IOP Conf. Series: Earth and Environmental Science 55 Conference 1 : 2017)
Bibliografi
Author:
Afifah, Diana Nur
;
Rustanti, Ninik
;
Anjani, Gemala
;
Syah, Dahrul
;
Yanti
;
Suhartono, Maggy Thenawidjaja
Topik:
JABFUNG-YANTI-2018
Bahasa:
(EN )
Penerbit:
IOP Publishing
Tempat Terbit:
Bristol
Tahun Terbit:
2017
Jenis:
Article - diterbitkan di jurnal ilmiah internasional
Fulltext:
2017_IOP_PROTEOMICS.pdf
(732.7KB;
4 download
)
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Abstract
This paper presents the proteomics study which includes separation, identification and characterization of proteins. The experiment on Indonesian fermented food such as extracellular fibrinolytic protease from Bacillus licheniformis RO3 and Bacillus pumilus 2.g isolated from red oncom and tempeh gembus was conducted. The experimental works comprise the following steps: (1) a combination of one- and two-dimensional electrophoresis analysis, (2) mass spectrometry analysis using MALDI-TOF-MS and (3) investigation using protein database. The result suggested that there were new two protein fractions of B. licheniformis RO3 and three protein fractions of B. pumilus 2.g. These result has not been previously reported.
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