| Phytochemical compounds from plants have been used as promising therapeutics to prevent and treat various diseases including cancer. Pithecellobium jiringa seed, known as jengkol, has possessed pharmacological effects, such as antioxidant, antimicrobial, and antiinflammatory activities. In this study, anticancer activity of polyphenol fractions derived from P. jiringa parts (peel, seed coat, and seed) was measured by modulating cell mortality and matrix metalloproteinases (MMPs) expression in A549 lung and MCF7 breast cancer cells using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), gelatin zymography, and Real Time-PCR assays. Polyphenol fraction from each part of P. jiringa was extracted in 70% ethanol and identified using pyrolysis-Gas Chromatography/Mass Spectrometry (Py-GC/MS). MTT profiles showed that polyphenol fractions from P. jiringa parts dose dependently increased cell mortality of A549 and MCF7 cancer cells. Gelatin zymographic profiles revealed that P. jiringa polyphenol fractions at lowest dose (5 µg mL-1) significantly decreased MMP-2 and MMP-9 activities secreted from A549 cells. At gene level, polyphenol fractions exhibited dosage effect on the decreased expression of MMP-2 mRNA in both A549 and MCF7 cancer cells. In summary, polyphenol fractions from P. jiringa parts may exert anticancer effect via attenuating MMP-2 expression in vitro. |