(n-3) Long-Chain PUFA Differentially Affect Resistance to Pseudomonas aeruginosa Infection of Male and Female cftr–/– Mice  

Oleh:

Tiesset, Helene ; Bernard, Henry ; Bartke, Nana ; Beermann, Christopher ; Flachaire, Elisabeth ; Desseyn, Jean-Luc ; Gottrand, Frederic ; Husson, Marie-Odile

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: JN: The Journal of Nutrition vol. 141 no. 06 (Jun. 2011), page 1101-1107 .
Topik: Pseudomonas aeruginosa

Ketersediaan

Perpustakaan FK Nomor Panggil: J42.K.2011.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The aim of this study was to determine whether oral supplementation with EPA/DHA (10.5 and 5.1% of fat, respectively) could improve the outcome of pulmonary P. aeruginosa infection in cftr–/– mice compared with wild-type (Wt) mice similarly treated. Because gender could influence the susceptibility of cftr-deficient mice, results were analyzed by gender. Wt and –/– mice were randomized for 6 wk to consume a control or EPA/DHA diet, infected with endotracheal injection of 5 × 107 CFU/mouse of P. aeruginosa, and killed 24 h later. Cftr–/– mice were more susceptible to infection than were Wt mice; –/– males had more neutrophils (P < 0.01) and a higher keratinocyte-derived chemokine (KC) level (P < 0.05), and –/– females had greater lung injury and mortality (P < 0.05). Female –/– mice were more susceptible than –/– males with a higher mortality and lung injury (P < 0.05). The EPA/DHA diet reduced neutrophil numbers and KC and IL-6 levels (P < 0.05) in –/– males and reduced mortality rate (P < 0.001), lung permeability, and IL-6 level (P < 0.05) in –/– females compared with –/– mice fed the control diet. These results were associated with a reduction in the pulmonary bacterial load (P < 0.05), an increase in the EPA/DHA concentration in cell membranes of –/– males and females (P < 0.01), and an increased weight gain only in males compared with –/– mice fed the control diet (P < 0.01). In conclusion, EPA/DHA improves the host resistance of –/– mice, although the beneficial effect differed in males and females.

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