Heparin inhibits interferon-y signaling in human endometrial stromal cells by interference with the cellular binding of interferon-y  

Oleh:

Fluhr, Herbert ; Spratte, Julia ; Heidrich, Stephanie ; Ehrhardt, Jens ; Steinmuller, Frauke ; Zygmunt, Marek

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 95 no. 04 (Mar. 2011), page 1272-1277.
Topik: ENDOMETRIAL; Heparin; low molecular weight heparin; IFN-?; STAT-1; IRF-1; Nmi; endometrium

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To examine the impact of heparins on interferon-? (IFN-?) signaling in human endometrial stromal cells (ESCs) in vitro. Design In vitro experiment. Setting Research laboratory at a medical university center. Patient(s) Premenopausal women undergoing hysterectomy for benign reasons. Intervention(s) The ESCs were isolated from hysterectomy specimens, decidualized in vitro using P and 17ß-E2, and incubated with recombinant IFN-?, unfractionated heparin, and low molecular weight heparins (LMWHs). Main Outcome Measure(s) Interferon response factor 1 (IRF-1) and N-myc interactor (Nmi) messenger RNA (mRNA) were measured using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Phosphorylation of signal transducer and activator of transcription 1 (STAT-1) was detected by an in-cell Western assay, expression of the IFN-? receptor by flow cytometry. Cell-bound IFN-? was determined in lysates by an ELISA. Result(s) Heparin and LMWHs inhibit the IFN-?-mediated induction of IRF-1, but not Nmi in undifferentiated and decidualized ESCs. The phosphorylation of signal transducer and activator of transcription 1 STAT-1 upon IFN-? stimulation is inhibited as well. Heparin has no effect on the IFN-? receptor in ESCs, but inhibits the binding of IFN-? to the cells. Conclusion(s) Unfractionated heparin, as well as LMWHs, are able to inhibit IFN-? signaling in human ESCs and therefore might be clinically interesting agents to modulate the actions of this proinflammatory cytokine at the implantation site.

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