Assessing loss of imprint methylation in sperm from subfertile men using novel methylation polymerase chain reaction Luminex analysis  

Oleh:

Sato, Akiko ; Hiura, Hitoshi ; Hiroaki, Okae ; Miyauchi, Naoko ; Abe, Yukiko

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 95 no. 01 (Jan. 2011), page 129-134.
Topik: Bisulfite PCR-Luminex methylation analysis; BPL; DNA methylation; genomic imprinting; oligozoospermia; sperm

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2011.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To assess the clinical value of bisulfite polymerase chain reaction Luminex (BPL), an automated, high-throughput procedure for the detection of alterations in DNA methylation. Design Experimental prospective study. Setting University research laboratory and private in vitro fertilization (IVF) clinic. Patient(s) A total of 337 men, 61 with severe oligozoospermia, 67 with moderate oligozoospermia, and 209 with microscopically normozoospermia. Intervention(s) The ejaculated sperm samples after the routine semen analysis with patients' consent. Main Outcome Measure(s) Examination of the methylation patterns of eight imprinted loci in sperm DNA, and confirmation with combined bisulfite PCR restriction analysis (COBRA). Result(s) A total of 47 cases (13.9%) showed abnormal methylation at one or more imprinted loci (18 paternal, 18 maternal, and 11 cases with alterations of both maternal and paternal imprints). Conclusion(s) The relative ease of the BPL method provides a practical method within a clinical setting to reduce the likelihood of abnormal samples being used in assisted reproduction treatments.

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