Investigation of Propofol Concentrations in Human Breath by Solid-phase Microextraction Gas Chromatography–Mass Spectrometry  

Oleh:

Gong, Y. ; Li, E.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The Journal of International Medical Research vol. 37 no. 05 (Sep. 2009), page 1465-1471.
Topik: anaesthesia; propofol; exhaled gas; plasma; solid-phase microextraction; gas chromatography; mass spectrometry
Fulltext: 1465.pdf (108.98KB)

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Perpustakaan FK Nomor Panggil: J11.K.2009.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Propofol has been detected in human breath after being used as an intravenous anaesthetic, and this could provide a non-invasive method for monitoring propofol anaesthesia. The physicochemical properties of propofol allow it to diffuse across the alveolocapillary membrane and to be prepared as a calibration gas. In this study, headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC–MS), coupled with an external standard, was applied to assess propofol levels in the breath and plasma from three subjects under intravenous anaesthesia. Lower quantitation limits were 3.6 ng/l and 0.2 mg/l for propofol analysis in breath and arterial plasma, respectively. Intraday precision and recovery percentages for propofol detection in breath were 4.3 – 6.7% and 98 – 108%, respectively, and in plasma they were 3.8 – 6.1% and 90.1 – 125.1%, respectively. Propofol concentrations were 4.3 – 33.5 ng/l in breath and 3.2 – 6.8 mg/l in arterial plasma. A correlation was shown between propofol concentration in breath and plasma. Thus, HS-SPME-GC–MS, coupled with an external standard, could be a reliable and sensitive analytical technique for detecting propofol in breath during anaesthesia.

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