Expression And Genotype Of Manganese Superoxide Dsimutase In Lung Cancer Cells Of Smoker Patients  

Oleh:

Wanandi, Septelia Inawati ; Syahruddin, Elisna

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Makara Seri Kesehatan vol. 13 no. 2 (Dec. 2009), page 83-87.
Topik: enzyme specific activity; genotype; gene expression; lung cancer; MnSOD; smoker

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM75.2 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Although the most common cause of lung cancer is long-term exposure to tobacco smoke, the role of genetic factor for the cell defense mechanism against lung cancer, such as MnSOD, should also be considered. MnSOD is the major antioxidant in matrix mitochondria, which plays a pivotal role in eliminating anion superoxide free radical generated from the tobacco smoke. The genotype of human MnSOD which is related to various cancers has been reported, but the correlation between the genotype and the enzyme activity remains unknown. The aim of this study is to analyze the expression (mRNA and enzyme specific activity) and genotype of MnSOD in lung cancer cells of smoker patients. Samples were normal and lung cancer cells of patients operated in Persahabatan Hospital from May to December 2008 (3 patients), as well as lung cancer cells extracted from FFPE collection of Persahabatan Hospital. Leukocyte cells of 15 healthy subjects aged more than 40 years with a history as a smoker for more than 10 years were used as controls. MnSOD gene expression was analyzed based on relative mRNA level and specific activity of MnSOD in lung cancer and leukocyte cells. The genotyping was performed using PCR-RFLP. The result showed that the lung cancer cells

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