Detail Characterization of an immunologically conserved epitope from hepatitis C virus E2 glycoprotein recognized by HLA-A2 restricted cytotoxic T lymphocytes (from Journal of Hepatology 2001, 34, 321-329) Bibliografi Author: Sarobe, Pablo ; Huarte, Eduardo ; Lasarte, Juan Jose ; Cerio, Ascension Lopez-Diaz de ; Borras-Cuesta, Francisco ; Prieto, Jesus Topik: Hepatitis C virus; Cytotoxic T lymphocytes; Epitope; HLA binding; Validation ref - 6 Bahasa: (EN )     Penerbit: Elsevier     Tahun Terbit: 2001     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: sarobe2001.pdf (292.57KB; 0 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract Background/Aims: Identi®cation of epitopes recognized by cytotoxic T lymphocytes (CTL) in hepatitis C virus (HCV) proteins is of importance because they can be used for vaccination, treatment of infection or monitoring of immune responses. Our purpose was to characterize new CTL epitopes in HCV structural proteins. Methods: Peptides were synthesized and tested in HLA-A2 binding assays. Binder peptides were used to stimulate peripheral blood mononuclear cells from HCV1 patients and controls, and activity measured in chromium release and ELISPOT assays. Results: Twenty binder peptides were found, and stimulation of HCV1 patient cells with nine peptides showing high binding ability led to the growth of CD81 CTL recognizing peptide E2(614-622) in association with HLA-A2. Peptide E2(614-622) was recognized by 30% of HLA-A21 patients with chronic HCV infection, but no responses were observed in control groups. Five peptides derived from region E2(614-622) from 26 different viral isolates bound to HLA-A2 molecules, and all of them but one, containing Phe at position 622, were recognized by E2(614-622) speci®c CTL. Conclusions: These results show that peptide E2 614-622) belongs to a highly conserved region of HCV E2, and might be a good candidate to induce anti-HCV CTL responses in HLA-A21 subjects. [validation ref - 6] Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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