Short Communication Single Strand Conformation Polymorphism Method for Initial Detection DNA Sequences Homogeneity  

Oleh:

Hidayat, Topik ; Pancoro, Adi

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 17 no. 1 (Mar. 2010), page 50-52.
Topik: Homogenous Sequences; Internal Transcribed Spacer Region; Molecular Phylogenetic; SSCP Method
Fulltext: 167.pdf (47.06KB)

Isi artikel

In molecular phylogenetic study, homogeneity of DNA sequences is a prerequisite before putting it into practice. Internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) has been common in phylogenetic study, but a homogenous sequence is often difficult to obtain. Here we use single-stranded conformation polymorphism (SSCP) method to detect homogeneity for nine pooled amplified products of ITS region. Our results suggested that SSCP method has been applicable in detection homogeneity of ITS region prior to using it in sequencing processes.

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