Intracellular Ca2+ Regulation in Calcium Sensitive Phenotype of Saccharomyces Cerevisiae  

Oleh:

Hermansyah

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 17 no. 1 (Mar. 2010), page 1-4.
Topik: Saccharomyces Cerevisiae; Protein Phosphatase; Calcium Sensitivity; Calcium Accumulation
Fulltext: 158.pdf (86.64KB)

Isi artikel

Intracellular cytosolic Ca2+ concentration accumulation plays an essential information in Saccharomyces cerevisiae i.e. to explain cellular mechanism of Ca2+ sensitive phenotype. Disruption both S. cerevisiae PPase PTP2 and MSG5 genes showed an inhibited growth in the presence of Ca2+. On the other hand, by using Luminocounter with apoaequorin system, a method based upon luminescent photoprotein aequorin, intracellular Ca2+ concentration was accumulated as a consequence of calcium sensitive phenotype of S. cerevisiae. This fact indicated that PPase ptp2? and msg5? were involved in intracellular Ca2+ transport in addition their already known pathways i.e Mitogen Activated Protein Kinase cell wall integrity pathway, high osmolarity glycerol (HOG) pathway, and pheromone response FUS3 pathway.

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