Effect of Oxygenated Water and Probiotic Administration on Fecal Microbiota of Rats  

Oleh:

Surono, Ingrid Suryanti ; Khomsan, Ali ; Sobariah, Enok ; Nurani, Darti

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 4 no. 1 (Apr. 2010), page 17-21.
Topik: Oxygenated water; probiotic; in vivo; viable; fecal microbiota; dadih
Fulltext: 4.PDF (3.07MB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM78 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Oxygenated water is with increased concentration of physically dissolved oxygen, and can perform all the same fungtions as the oxygen absorbed through the lungs. Several structures of human organs participate in the absorption and transportations of the oxygen, including the villi and cells containing mitochondrion in the amall intestine as well as the lymph system. The aim of this in vivo study were there folds, to validate the support of oxygenated water on viability of probiotic bacteria in the GUT, to suppress the fecal coliform, and to study the effect of oxygen concentration on the profile of facel microbiota. There were one control group and three probiotic groups of 5 rats each based on strain of probiotic suplementation, control without probiotic, Lactobacillus Casei commercial strain, Lactobacillus sp IS-7257 and Lactobacillus sp IS-27560. Each group was treated with three variable treatments, without oxygenated water supplementation, supplemented with oxygenated water at 50 ppm, and at 80 ppm, Facel samples were collected before, after 3 days, 7 days, supplementation, followed by 3 days after returning back to normal diet, analysed by culture dependent analyses for viable fecal lactic, coliform and fecal anaerobic bacteria. Supplementation of oxgenated water at 50 ppm, significantly increase fecal lactic acid bacteria of all probiotic groups after 3 and 7 days (P<0.05); 80 ppm oxygenated water tends to lower the fecal coliform (P<0.1), while oxygenated water administration gives no effect on fecal anaerobic bacteria. As a conclusion, 50 ppm oxygenated water administration significantly increased viable fecal lactic acid bacteria in probiotic groups. On the other hand, 80 ppm oxygenated water administration tends to lower the fecal coliform bacteria. No effect of administration probiotic and/or oxygenated water on viability of facal anaerobic bacteria.

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