Detail Comparison of FBS and FBS substitute containing media in umbilical cord blood stem cell cultures: A preliminary study (article of Biochemistry - an Indian Journal Vol.7 No.1) Bibliografi Author: Pawitan, Jeanne Adiwinata ; Bustami, Arleni ; Liem, Isabella Kurnia ; Budiyanti, Ecie ; Sumapradja, Kanadi Topik: Umbilical cord blood; Mesenchymal stem cell; Platelet rich plasma; Human AB serum; Osteoclast-like cells; Ficoll gradient centrifugation Bahasa: (EN )     Penerbit: Trade Science Inc.     Tahun Terbit: 2013     Jenis: Journal - ilmiah internasional Fulltext: ecie_jurnal 1_comp FBS.pdf (316.64KB; 1 download) Abstract Aim: This study aimed to compare FBS and FBS substitute containing media in umbilical cord blood (UCB) derived stem cell culture. Experimental: Processing of UCB was done by Ficoll gradient or simple centrifugation, which was followed by lysis buffer treatment. The pellets were then cultured in FBS and FBS substitute containing media. Results: Attached cells were heterogeneous. There were fibroblastic cells and rounded cells of the macrophage-monocyte lineage. Ficoll gradient centrifugation and FBS containing DMEM, and simple centrifugation and VEGF and human AB serum supplemented DMEM yielded confluent cul- tures. However, after passage-1, the cells differentiated predominantly into osteoclast-like cells, cell growth was arrested, and further passage was impossible. Conclusion: UCB is a poor source of MSCs, therefore, Ficoll gradient and simple centrifugation processing combined with FBS or FBS substitute containing media are not suitable methods for the isolation and culture of UCB derived MSCs. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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