Background : Mortality rate of Staphylococcus aureus infection reach 50%, meanwhile prevalence of Staphyloccus aureus infection in Indonesia is 23,5%. Staphylococcus aureus infection therapy can be done using antibiotics, but sometimes it became resistant. Neem (Azadirachta indica) is a tree that comes from India and has flavonoid which has antibacterial effect.
Purpose : The purpose of this study is to know the antibacterial potential in neem leaf extract against Staphylococcus aureus.
Methods : This is an experimental in vitro studies. Neem leaf powder was from Institut Pertanian Bogor and extracted using Soxhlet in methanol. Extract was made into 4 different concentration : 0,6 mg/mL, 1,2 mg/mL, 2,4 mg/mL, dan 4,8 mg/mL. Antimicrobial susceptibility test was done against Staphylococcus aureus ATCC 6538 using Broth Macro Dilution Test to measure MIC value. Pour plate was done to measure which concentration that kills =99,99% colonies. Statistical test was done using One way Anova test and Post Hoc Anova Test.
Result : MIC couldn’t be assessed because the extract was turbid. The concentration that kills =99,99% colonies in this experiment is 2,4 mg/mL. There is significant difference of colonies in control MB (Media and Bactery) against experimental group of extracts (P=0,001). There is no significant difference between group of extracts against different concentration (P1= 0,999 , P2=1,000, P3=1,000).
Conclusion : Neem has antibacterial potential. Minimal concentration to kill =99,99% colonies is 2,4 mg/mL. The number of colony decreased with increasing concentration of extract. |