Physiological Characterization and Molecular Identification of Denitrifying Bacteria Possesing Nitrous Oxide High Reduction Activity Isolated from Rice Soils  

Oleh:

SETYANINGSIH, RATNA ; Rusmana, Iman ; SETYANTO, PRIHASTO

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 04 no. 02 (Aug. 2010), page 75-78.
Topik: denitrifying bacteria; N2O reduction; rice soils
Fulltext: Physiological Characterization_Ros.pdf (5.3MB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM78 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk
Perpustakaan FK Nomor Panggil: M53.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk

Isi artikel

Rice fields are one of the main sources of high nitrous oxide N2O emission. Soil denitrifiers possessing high N2O reduction activity are important for controlling N2O emission. Nitrous oxide reduction is the last step of denitrification process. The aims of this study were to characterize and to identify denitrifying bacteria isolated from rice soils possessing high activity of N2O reduction. Soil samples were taken from 6 locations of rice fields in Bogor (West Java) and Tangerang (Banten), Indonesia. Physiological characterization was performed using API 20 NE, while molecular identification was conducted based on the 16S rRNA gene sequence. It was found that ten isolates of denitrifying bacteria were able to grow using N,O as an electron acceptor as indicated by decreasing N2O concentration in the headspace of the cultures. The bacterial growth indicated by optical density, increased up to 0.12-0.47 after 5 days incubation. Isolate BL2 had the highest activity of N,O reduction followed by BL1 and BLN 1 at up to 5.41,4.09, and 3.91 µmol mL-1 bacterial cultures, respectively. The BL1, BL2, and BLN 1 isolates had some different physiological characteristics. Based on their 16S rRNA sequence, BL 1 and BLN 1 were closely related to Ochrobactrum anthropi ATCC 49188 with similarity of 99%.

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