Validation of a novel computer-assisted sperm analysis (CASA) system using multitarget-tracking algorithms  

Oleh:

Tomlinson, Mathew James ; Pooley, Karen ; Simpson, Tracey

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 93 no. 06 (Apr. 2010), page 1911-1920.
Topik: CASA validation; motility; sperm concentration; hemocytometer

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To determine the accuracy and precision of a novel computer-assisted sperm analysis (CASA) system by comparison with existing recommended manual methods. Design Prospective study using comparative measurements of sperm concentration and motility on latex beads and immotile and motile sperm. Setting Tertiary referral fertility center with strong academic links. Patient(s) Sperm donors and male partners of couples attending for fertility investigations. Intervention(s) None. Main Outcome Measure(s) Achievement of Accubead target value for high and low concentration suspensions. Repeatability as demonstrated by coefficients of variation and intraclass correlation coefficients. Correlation and limits of agreement between CASA and manual methods. Result(s) The CASA measurements of latex beads and sperm concentrations demonstrated a high level of accuracy and repeatability. Repeated Accubead measurements attained the required target value (mean difference from target of 2.61% and 3.71% for high- and low-concentration suspensions, respectively) and were highly reproducible. Limits of agreement analysis suggested that manual and CASA counts compared directly could be deemed to be interchangeable. Manual and CASA motility measurements were highly correlated for grades a, b, and d but could not be deemed to be interchangeable, and manual motility estimates were consistently higher for motile sperm. Conclusion(s) The novel CASA system was able to provide semen quality measurements for sperm concentration and motility measurements which were at least as reliable as current manual methods.

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