Numerical chromosome abnormalities are associated with sperm tail swelling patterns  

Oleh:

Myung-Geol, Pang ; Young-Ah, You ; Yoo-Jin, Park

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 94 no. 03 (Aug. 2010), page 1012-1020.
Topik: Sperm aneuploidy; FISH; hypo-osmotic swelling; male infertility

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To assess whether the differential sperm tail swelling patterns observed following hypo-osmotic shock are useful in discriminating normal sperm from aneuploid sperm. Design Prospective study. Setting University research setting. Intervention(s) Fluorescence in situ hybridization (FISH) was combined with hypo-osmotic swelling for a simultaneous assessment of aneuploidy and viability in human spermatozoa. Main Outcome Measure(s) FISH for chromosomes 1, 13, 18, 21, X, and Y after hypo-osmotic stress was used to investigate the distribution of sperm aneuploidy related to sperm-tail swelling patterns. A total of 16,473 sperm cells were scored from three normal fertile donors and six oligoasthenoteratozoospermic (OAT) patients. Result(s) There was a 17.2-fold decrease in the frequency of total aneuploidy in the sperm with a tail-tip swelling pattern compared with the initial nonselected sperm in the OAT patients. Strikingly, when the sperm with tail-tip swelling patterns were screened from the patients, the frequency of total aneuploidy was actually lower by a factor of four than in the nonselected sperm from fertile donors. Conclusion(s) The sperm cells with tail-tip swelling patterns are related to a low frequency of aneuploidy.

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