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BukuPrevalence of Vibrio cholera from Refilled Drinking Water in Jakarta, Indonesia (presented at ICBFE 2010: International Conference on Biotechnology and Food Engineering, Singapore 25-27 August 2010)
Bibliografi
Author: Waturangi, Diana Elizabeth ; Kristyanto, Sylvia
Topik: V. cholerae; drinking water; most probable number; multiplex PCR; JABFUNG-DEW-2015-19
Bahasa: (EN )    
Penerbit: World Academy of Science, Engineering and Technology (WASET)     Tempat Terbit: Singapore    Tahun Terbit: 2010    
Jenis: Papers/Makalah - pada seminar internasional
Fulltext:
Abstract
V. cholerae is a fecal coliform organism which is responsible for life-threatening diarrhea known as cholera. This organism is an indicator microorganism whose presence indicates that the water may be contaminated that can cause disease. Drinking water, including refilled drinking water, may be contain some contaminants such as V. cholerae. A total of 50 samples from refilled drinking water were examined in this study by using combination between most probable number (KM) and polymerase chain reaction (PCR). The prevalences of V. cholerae were 0.0% for North and West Jakarta, and 10.0% for East, South, and Centre Jakarta. The maximum numbers of V. cholerae in refilled drinking water were 460 MPN/mL from Centre Jakarta. Based on the results of the multiplex PCR, three samples of water gave positive results (6.0%) and from the positive water samples we isolated 19 isolates. 16 of the isolates (84.2%) were positive for toxR, 8 of the isolates for ctxA and ace (42.1%), 3 of the isolates for tcpA, 1 of the isolate for zot, and none for ompU. This results indicated there are still contamination of V. cholerae.
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