Detail Prevalence and level of Vibrio cholerae from street foods using most probable number and multiplex PCR (presented at 4th International Biochemistry and Molecular Biology Conference 2014 Bangkok, Thailand 2-3 April 2014) Bibliografi Author: Waturangi, Diana Elizabeth Topik: street foods; Vibrio cholerae; ice; beverages; fresh fruits; vegetables; JABFUNG-DEW-2015-12 Bahasa: (EN )     Penerbit: Department of Biochemistry, Faculty of Science, Kasetsart University, Thailand     Tempat Terbit: Bangkok, Thailand    Tahun Terbit: 2014     Jenis: Papers/Makalah - pada seminar internasional Fulltext: B-12_Diana Waturangi.pdf (4.87MB; 6 download) Prevalence and level of vibrio jafung b12.pdf (55.32KB; 5 download) Abstract Street foods are ubiquitous throughout Indonesia and are a vital source for food among Indonesian people. Unfortunately it can be major source food and waterborne diseases including V. cholerae. Inadequate safety measures in drinking-water supplies, improper sanitation and poor hygiene of fruits and fresh vegetables can increase the risk of cholera outbreaks. In this study, we have detected and enumerated V. cholerae from ice, beverages, fresh fruits and vegetables collected from several areas comprising five different regions of Jakarta, Indonesia. Enumeration of V. cholerae was determined by using the three-tube MPN method, and for ice and beverages ranged from <0.3 to >110 MPN/mL. The presence of regulatory and virulence gene sequences were determined by using uniplex/multiplex polymerase chain reaction (PCR). Out of 110 samples tested, 33 (30%) yielded positive for V. cholerae, 21 (64%) of which were ice samples and the remaining 12 (36%) were beverages. A total of 88 V. cholerae strains were isolated, based on the presence of toxR gene sequence by PCR, followed by determining the presence of other genetic markers, such as, hlyA (59%), ompU (16%) and ctxA (19%), While for fuits and vegetables we found from 3 fruits and 2 vegetables were positive for toxR gene, in search of potential pathogens and their patterns. The detection and isolation of potentially harmful V. cholerae indicate that these products are not devoid of risk from choleragenic vibrios, particularly due to the emergence of classical biotypes of V. cholerae O1 and potentially harmful non-O1 serovars of this species. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

Copyright © 2006, 2007 Unika Atma Jaya, all rights reserved