Determination of L-type calcium current in the primary hypertrophied left ventricular cardiomyocytes of hypertrophy heart rats/HHR  

Oleh:

Danes, Vennetia R.

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI - non-atma jaya
Dalam koleksi: Media Medika Indonesiana vol. 43 no. 06 (Sep. 2009), page 269-276.
Topik: HHR rats; LV hypertrophy; cardiomyocyte; L-type calcium current

Ketersediaan

Perpustakaan FK Nomor Panggil: M30.K.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Background: The molecular mechanisms responsible for electromechanical derangements found in primary left ventricular (LV) hypertrophy have not been clearly elucidated. Of particular interest is the voltage gated L-type Ca current (ICa L) which plays crucial role in the EC-coupling. This study aimed to investigate cardiomyocyte ICa(L) in newly developed line of rats namely HHR (hypertrophy heart rat) and NHR (normal heart rat). Methods: A pure experimental study has been conducted using male HHR (n=7 rats) and NHR (n=9 rats), aged 19-25 week. Cardiomyocytes were isolated enzymatically. By using whole cell patch clamp techniques, ICa were measured and normalized (pA/pF) for cell size determined by capacitance (Cm). In Na-free superfusate, the ICa(L) of HHR (n=18 cells) and NHR (n=12 cells) obtained at holding potential (HP) -50 mV were analyzed using Student's t-test and one-way ANOVA (repeated measures). p<0.05 values were considered significance. Results: Myocyte mean Cm of HHR slightly increased but not significantly greater than that of NHR (364.3±22.33 pF vs 300.2±27.78 pF, p=0.08). In HHR, peak Ca current density was significantly increase, i.e, at HP -50 mV, the ICa(I.) evoked at test potential (TP) -10 m V were -7.7 4±0.86 (HHR) vs -4. 68±1. 09 (NHR) pA/pF, p

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