PERBANDINGAN KADAR HEMOGLOBIN ANTARA METODE SPECTROPHOTOMETER DENGAN METODE HEMOCUE PADA SAMPEL LEUKOSITOSIS  

Oleh:

Andriyoko, Hasti ; Lismayanti, Leni ; Prihatni, Delita

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Indonesian Journal of Clinical Pathology and Medical Laboratory vol. 15 no. 03 (Jul. 2009), page 109-110.
Topik: Haemoglobin; specthrophotometer; HemoCue method; leucocytosis

Ketersediaan

Perpustakaan FK Nomor Panggil: I01.K.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Background and objective: The measurement of haemoglobin was carried out by using hematology analyzer with specthrophotometer method which measured light absorbance at 540 nm. However, this measurement affected by increase turbidity as a result of leucocytosis. The turbidity can be eliminated by using HemoCue method that detect absorbance at 570 nm and 880 nm. The aim of this study was to compare the measurement of haemoglobin obtained between specthrophotometer method and HemoCue method. Materials and method: Blood EDTA sample that have been measured with MEK-6318K Nihon Kohden hematology analyzer for haemoglobin levels with spectrophotometer methods with leucocyte > 100.000/mm3 were included in this study. Blood sample are measured again for the haemoglobin level with HemoCue B-Haemoglobin Analyzer. This study was conducted at Clinical Pathology Laboratory, Dr. Hasan Sadikin Hospital Bandung from August-October 2008. Result: Seventeen leucocytosis sample were enroled in this study. The mean haemoglobin level from specthrophotometer method is higher than HemoCue method, however there was no statistically significant difference between haemoglobin result from specthrophotometer method and HemoCue method (p = 0.742 > a = 0.05). Conclusion: There was no significant difference beetween speethrophotometer method and HemoCue method for haemoglobin measurement of leucocytosis sample.

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