Aquaporin-4 deficiency induces subfertility in female mice  

Oleh:

Xiu-Lan, Sun ; Jing, Zhang ; Yi, Fan

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 92 no. 05 (Nov. 2009), page 1736-1743.
Topik: Aquaporin 4; knockout; reproduction; female; mice

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To investigate the effects of aquaporin-4 (AQP4) on female fertility. Design Prospective, randomized animal study. Setting Medical university. Animal(s) AQP4+/+ and AQP4-/- female mice. Intervention(s) Eight- to 10-week-old virgin female AQP4+/+ and AQP4-/- mice were subjected to a mating study. Superovulation was carried out on 4-week-old AQP4+/+ and AQP4-/- mice. The protocol consisted of a single intraperitoneal (IP) injection with 5 units of pregnant mare serum gonadotropin (PMSG) for 48 hours, followed by 5 units of human chorionic gonadotropin (hCG) for an additional 14 or 48 hours. Main Outcome Measure(s) Pregnancy success, oocyte count, morphological changes in ovaries and uteri, and alterations of estrous cycle. Result(s) AQP4-null mice displayed subfertility as evidenced by a lower rate of pregnancy and decreased litter size. Further studies revealed that in AQP4-null mice, there were fewer numbers of antral follicles and corpora lutea in the ovaries and that uteri presented as hyporeactive to gonadotropins. In addition, in AQP4-null mice, superovulation induced by hCG and PMSG yielded fewer numbers of antral follicles, atretic follicles, corpora lutea, and oocytes in ovaries, and uteri exhibited a decreased response to exogenous gonadotropins. Conclusion(s) AQP4 deficiency induces subfertility in female mice.

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