Detection of Restricted Junctional Diversity of Peripheral T Cells in SLE Patients by Spectratyping  

Oleh:

Kolowos, W. ; Herrmann, M. ; Ponner, BB ; Voll, R. ; Kern, P. ; Frank, C.R. ; Kalden, JR

Jenis: Article from Journal
Dalam koleksi: Lupus vol. 6 no. 9 (1997), page 701-707.
Topik: Spectratyping; TCR Beta; -Chain; Oligoclonal T cell Expansion
Fulltext: 701.full.pdf (627.28KB)

Isi artikel

Analysis of somatic mutations revealed that anti-double-stranded DNA (dsDNA) autoantibodies from patients with systemic lupus erythematosus (SLE) share features of a T cell dependent, antigen driven immune response. Therefore we analysed the length diversity of the complementarity determining region 3 (CDR3) of T cell receptor (TCR) by high resolution gel electrophoresis of 16 Vβ family specific RT PCR products (spectratyping). To enable statistical analysis we developed a quantitative scoring method for the histograms. We investigated 16 Vβ gene families in peripheral T cells of SLE patients (n = 9) with active (n = 5) and inactive (n = 4) disease as well as normal healthy blood donors (NHD; n = 9). Analysis of TCR Vβ spectratypes (active SLE, n = 59; inactive SLE, n = 51 and NHD n = 97) revealed statistically significant differences of CDR3 length distribution between SLE patients and NHD (P < 0.0001 (active SLE/NHD) and P = 0.0034 (inactive SLE/NHD). These results suggest that spectratyping is able to detect clonal activation of peripheral T cells which correlates to disease activity in SLE patients. We conclude that peripheral T cells from SLE patients display features of a secondary antigen driven immune response.

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