Detail Biochemical Characterization of ADP-Ribose Polymer Metabolism in SLE   Oleh:  Chen, H-Y ; Pertusi, RM ; Kirkland, JB ; Robin, BR ; Jacobson, EL Jenis: Article from Journal Dalam koleksi: Lupus vol. 5 no. 1B (1996), page 14-21. Topik: Poly(ADP-Ribose) Polymerase; Poly(ADP-Ribose) Glycohydrolase; NAD Fulltext: 14.full.pdf (686.13KB) Isi artikel The metabolism of poly(ADP-ribose) in peripheral blood mononuclear (PBM) cells was studied in 13 patients with systemic lupus erythematosus (SLE) and in 12 age and sex matched controls. Poly(ADP-ribose) polymerase activity was measured as the net accumulation of ADP-ribose polymers during the conversion of 3P-N2AD to poly(ADP-ribose) in PBM cells in vitro. The control population showed a mean activity of 418 ± 91(s.d.) pmol A6 DP-ribose/10 min/10 cells. The SLE population was more heterogeneous and showed a lower mean of 225 ± 147(s.d.) pmol ADP-ribose/ 10 m6 in/10 cells. The mechanism of decreased ADP-ribose polymer accumulation was investigated. Measurements of turnover of the ADP-ribose polymers and its substrate, N+,AD showed that diminished ADP-ribose polymer accumulation in SLE subjects resulted from decreased poly(ADPribose) synthesis and not from altered rates of polymer turnover or NAD utilization. Western blot analyses of enzyme protein levels, kinetic studies of poly(ADP-ribose) polymerase activity and analyses of polymer size distribution suggested that the mechanism of poly(ADP-ribose) synthesis in SLE cells is not altered but that the number of active poly(ADP-ribose) polymerase molecules is reduced. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Kembali

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