Detail MBP-1 Upregulates MiR-29b, Which Represses Mcl-1, Collagens, And Matrix Metalloproteinase-2 In Prostate Cancer Cells   Oleh:  Steele, Robert S. ; Mott, Justin L. ; Ray, Ratna B. Jenis: Article from Article Dalam koleksi: Genes and Cancer vol. 1 no. 4 (May 2010), page 381-387. Topik: MicroRNA; Prostate Cancer; Mcl-1; Extracellular Matrix Molecules Fulltext: 381.full.pdf (296.5KB) Isi artikel c-myc promoter binding protein (MBP-1) is a multifunctional protein known to regulate expression of targets involved in the malignant phenotype. We have previously demonstrated that exogenous expression of MBP-1 inhibits prostate tumor growth, although the mechanism of growth inhibition is not well understood. We hypothesized that MBP-1 may modulate microRNA (miRNA) expression for regulation of prostate cancer cell growth. In this study, we demonstrated that exogenous MBP-1 upregulates miR-29b by 5- to 9-fold in prostate cancer cells as measured by real-time quantitative reverse transcription polymerase chain reaction. Subsequent studies indicated that exogenous expression of miR-29b inhibited Mcl-1, COL1A1, and COL4A1. Furthermore, a novel target with potential implications for invasion and metastasis, matrix metallopeptidase-2 (MMP-2), was identified and confirmed to be a miR-29b target in prostate cancer cells. Together, these results demonstrated that exogenous expression of miR-29b regulates prostate cancer cell growth by modulating antiapoptotic and prometastatic matrix molecules, implicating the therapeutic potential of miR-29b for prostate cancer inhibition. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Kembali

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