Simultaneous determination in situ of DNA fragmentation and 8-oxoguanine in human sperm  

Oleh:

Santiso, Rebeca ; Tamayo, María ; Gosalvez, Jaime ; Meseguer, Marcos ; Garrido, Nicolas ; Fernandez, Jose Luis

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 93 no. 01 (Jan. 2010), page 314-318.
Topik: Sperm chromatin dispersion test; SCD; DNA fragmentation; DNA damage; oxidative DNA damage; 8-oxoguanine; reactive oxygen species

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Deoxyribonucleic acid fragmentation and oxidative DNA damage were simultaneously determined in the same sperm cell, incubating with an 8-oxoguanine DNA probe on human spermatozoa processed by the sperm chromatin dispersion test. The assay was validated by incubation with agents that induce DNA fragmentation with or without oxidative base damage. In all samples examined, increased levels of 8-oxoguanine were present only in those spermatozoa with fragmented DNA, suggesting a link between both DNA damage types.

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