Role of estrogen receptors in menstrual cycle–related neoangiogenesis and their influence on endothelial progenitor cell physiology  

Oleh:

Foresta, Carlo ; Toni, Luca De ; Mambro, Antonella Di ; Ferlin, Alberto ; Perilli, Lisa

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 93 no. 01 (Jan. 2010), page 220-228.
Topik: Endothelial progenitor cell; estrogen receptor; CXCR4; MMP9; menstrual cycle

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To study whether estrogen receptors (ERs) are expressed in vitro and in vivo by female circulating endothelial progenitor cells (EPCs); and the role of ERs in the periodic vascular damage and repair that occurs during the menstrual cycle. Design Quantification of circulating progenitor cells, EPCs, and relative CXCR4+ fraction by flow cytometry. Quantification of plasma 17ß-E2 by electrochemiluminescent immunoassay. Expression of ERs by immunofluorescence and immunohistochemistry. Estrogen receptor, CXCR4, and matrix metalloproteinase 9 gene expression by reverse transcriptase–polymerase chain reaction and real-time polymerase chain reaction. Setting University clinic and academic research laboratory. Patient(s) Twelve young fertile women (aged 22–27 years) observed for 6 months, 10 postmenopausal women (aged 52–63 years), and 50 male control subjects (aged 24–61 years). Intervention(s) Blood (35 mL) was collected at each observation point. Main Outcome Measure(s) Correlation between 17ß-E2 exposure and neoangiogenesis markers. Result(s) Estrogen receptors are expressed both in cultured EPCs after prolonged estrogen stimulation and in circulating EPCs, such as in CD34+ cells in bone marrow. The number of ER-ß+ and CXCR4+ EPCs increased during the ovulatory phase, and this increase is probably mediated by ER-ß and matrix metalloproteinase 9. Conclusion(s) Estrogens play a key role in neoangiogenesis processes, such as endometrium recovery, and this mechanism involves both a central action (on bone marrow) and a cytokine-mediated peripheral one (on endothelium).

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