DETEKSI ANTIGEN VIRUS DENGUE PADA PROGENI VEKTOR DEMAM BERDARAH DENGAN METODE IMUNOHISTOKIMIA  

Oleh:

Widiarti ; Boewono, Damar Tri ; Widyastuti, Umi

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Buletin Penelitian Kesehatan: Bulletin of Health Studies vol. 37 no. 03 (Oct. 2009), page 126-136.
Topik: Transovarian Transmission; Imunohistochemical; Dengue Antigen Detection; J

Ketersediaan

Perpustakaan PKPM Nomor Panggil: B52 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk
Perpustakaan FK Nomor Panggil: B18.K.2009.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk

Isi artikel

Dengue infection has expanded to other geographic areas in Indonesia due to changes in human ecology and behavior. In many tropical and subtropical areas of the world this viruses are endemic and causes periodic or annual outbreaks of disease. Among the complex and multifactors responsible for the increase of dengue cases, the transovarian mechanism may play an important role. This research was conducted to investigate the posibility of transovarian transmission of dengue virus in the population of Aedes aegypti and Ae. albopictus in endemic areas of Central Java Province. Aedes aegypti and Ae. albopictus larvae were collected from the DHF endemic areas i.e., Semarang Municipality,Kendal, Tegal, Grobogan and Sukoharjo Regency. Aedes sp. larvae from endemic areas were reared to adults in Insectary of Vector and Reservoir Diseases Research and Development Unit Salatiga. Head squashes of either male or female of all Aedes sp. mosquito (Ae. aegypti and Ae. albopictus) were tested for presence of dengue antigen in the brain based on immunohistochemical (streptavidin-biotin-peroxidase-complex)techniques using monoclonal antibody against dengue antigen (I C7) produced by Gadjah Mada University. Results indicatedthatAe. aegyptipopulation(0,48%- 8,77%)from 6 distric of 5 endemic area (Ngemplak Simongan and Manyaran Distric, Semarang Municipality, Cepiringand Kaliwungu District Kendal Regency, Ngruki and Makamhaji Grogol District, Sukoharjo Regency) have brownish color in their brain, mean while others have blue color. It was concluded that the population of Ae. aegypti from 6 districof 5 endemic area have shown the transovarian transmission of dengue virus.Brownish color was also found on the Ae. albopictus from Manyaran DistrictSemarang Municipality, therefore it was proved to be a dengue vector. Therefore vector control activities should focus on larval stages using larvicide or regular house hold sanitation or clean-up campaigns. The 3 M slogan which in English translates to dry off: cover and bllrJ' must be added accordingly for getting rid of mosquito's eggs (brush off) H'hichare attached to the surface containers(3M + S); this ml/st he informed soon to the community.

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